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. 2019 Apr 2;2(2):e201900374. doi: 10.26508/lsa.201900374

Figure S3. Reduced GFRA1+ cells in Nox1 KO testes.

Figure S3.

(A) Immunohistochemical analysis of Nox1 KO testes using antibodies against phosphorylated MAPK14 (p-MAPK14) and markers for undifferentiated spermatogonia (GFRA1) or Sertoli cells (GATA4). Arrow indicates a cell expressing both antigens. Scale bar: 20 μm. (B, C) Quantification of cells expressing GFRA1 (B) or GATA4 (C). *P < 0.05 (t test). (D) Immunohistochemical analysis of Nox1 KO testes using antibodies against phosphorylated MAPK7 (p-MAPK7) and an undifferentiated spermatogonia marker (GFRA1). Arrow indicates a cell expressing both antigens. Scale bar: 10 μm. (E) Quantification of cells expressing GFRA1. At least 20 tubules were counted. Counterstaining using Hoechst 33342. *P < 0.05 (t test). Data information: in (B, C, E), data are presented as mean ± SEM.