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. 2019 Mar 27;10:562. doi: 10.3389/fimmu.2019.00562

Figure 4.

Figure 4

In vitro ADCC assays. ADCC assays with calcein-AM loaded Raji cells as targets and (A) PBMCs, (B) NK cells, and (C) freshly isolated monocytes from the same healthy donor [homozygous V/V (left) or heterozygous V/F (center and right) and E:T ratio = 10:1] as effector cells. (A–C) The level of the released calcein-AM was measured to detect target cell lysis after 4 h. Error bars correspond to standard deviations of triplicate samples. Statistical analysis was performed by two way ANOVA with Tukey's multiple comparisons test (ns: P > 0.05, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ****P ≤ 0.0001).