Fig. 6.

PGD₂ and LTE₄ modulated gene transcription and production of cytokines, chemokines, and surface receptors in cultured Tc2 cells. a Venn diagram and heat map showing significantly (p < 0.05) regulated genes for cytokines, chemokines and surface receptors (red for up-regulation and green for down-regulation) in Tc2 cells detected by microarray after treatments with PGD₂, LTE₄ or their combination. Three experimental replicates were prepared for each condition. b Up-regulated cytokine genes determined by using a PCRarray after the same treatments as those in (a). c, d The change of mRNA levels in cells measured with qPCR (c) and protein levels in the cell supernatants detected by using Luminex assays (d) for selected cytokines and chemokine after the same treatments as above. The mRNA levels in control samples were treated as 1-fold. e The cytokine production induced by PGD₂ and LTE₄ were inhibited by TM30089 and montelukast. *p < 0.05, **p < 0.005, ***p < 0.001, (n=1 for a; n=3 for b and c; n=6 for d; n=4 for e).