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. 2019 Apr 4;14(4):e0214954. doi: 10.1371/journal.pone.0214954

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© 2019 Glas et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — A. Relative fluorescence changes (ΔF/F) for the five example neurons depicted in Fig 2A and 2B as recorded with a miniaturized microscope (blue) and a two-photon microscope (red) during visual stimulation. Top: Blue and red marks indicate stimulus presentation for miniaturized microscopy and two-photon microscopy, respectively. Stimuli were presented in a pseudo-randomized order that was unique for each experiment. B. Average ΔF/F response amplitude to the preferred stimulus (p = 1.841·10–49, Spearman’s correlation) and ΔF/F signal-to-noise ratio of stimulus-induced calcium transients (p = 6.348·10–21, Spearman’s correlation) of all matched neurons (488 neurons, n = 8 mice) in miniaturized microscope recordings plotted against the respective values in two-photon microscope recordings. Scale bars, 1 ΔF/F (A, vertical), and 25 s (A, horizontal).