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Representative dot-plots of the flow cytometry analyses for one TB patient (upper panel) and one non-TB patient (lower panel). Ascites lymphocytes were in vitro cultured overnight in the absence (medium) or the presence of PPD, HBHA or ESAT-6, as indicated. IFN-γ-producing CD4+T cells are shown after a sequential gating of Forward Scatter (FSC)/Side scatter (SSC), single cells and CD3+T lymphocytes. The percentages of IFN-γ+ T lymphocytes in each condition are indicated.
