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. 2019 Apr 4;14(4):e0214333. doi: 10.1371/journal.pone.0214333

Fig 4. Percentages of IFN-γ-containing CD4+ ascites T lymphocytes induced by PPD, HBHA or ESAT-6.

Fig 4

Ascites lymphocytes were in vitro cultured overnight in the absence or presence of PPD, HBHA ESAT-6, and the percentages of IFN-γ-containing CD4+ T lymphocytes were analyzed by flow cytometry. The values shown are obtained by subtracting the values obtained in the absence of antigen stimulation from those obtained in response to mycobacterial antigens. Filled circles represent values from patients suffering from TB, whereas open circles represent values from non-TB patients. Dotted horizontal lines represent the cut-offs determined by ROC analysis comparing the values for TB patients with those for non-TB patients. Values obtained for TB and non-TB patients were compared by Mann-Whitney U test. ***, P<0,001.