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. 2019 Apr 4;14(4):e0214847. doi: 10.1371/journal.pone.0214847

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© 2019 Willms et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 4 — HCT116 p53^+/+ and HCT116 p53^-/- cells were stimulated either with TRAIL (100 ng/ml), Mapatumumab (10 μg/ml) or Lexatumumab (10 μg/ml) for 3 h. (A) Whole cell lysates were analyzed for the phosphorylation/activity status and overall expression of various proteins associated with TRAIL-mediated non-apoptotic signaling pathways by Western blot. The level of actin was determined in parallel and served as loading control. Blots are shown for one representative experiment out of three performed. The ratios of p-JNK/ JNK, p-p38/ p38 and p-ERK1/2/ ERK1/2 were analyzed by densitometry (B-D). Intensity of each band was normalized to the respective actin.