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. 2019 Apr 4;14(4):e0214847. doi: 10.1371/journal.pone.0214847

Fig 5. Impact of p53 status on TRAIL-R-mediated IL8 induction.

Fig 5

HCT116 cells, RKO cells and A549 cells were stimulated either with TRAIL (100 ng/ml), Mapatumumab (10 μg/ml) or Lexatumumab (10 μg/ml) for 3 h. Relative expression of IL-8 mRNA level (normalized to TBP) was analyzed by qPCR (A, C, E). Relative expression of IL-8 protein level was analyzed by ELISA (B, D, F). Thee independent experiments, each performed in triplicates ± SD (n = 3) are shown in (A, B, D). One representative experiment out of three performed, each performed in triplicates ± SD are shown in (C, E, F).