Fig. 3.
4-HHE impairs insulin-induced glucose uptake. Rat L6 muscle cells were treated with 4-HHE or 4-HNE and then stimulated with 100 nmol/l insulin for 20 min. 2-Deoxy-d-[3H]glucose uptake was measured as described in the Methods. (a) Glucose uptake in L6 muscle cells treated for 60 min with 50 μmol/l 4-HHE or 4-HNE. Data are means±SEM, n = 5. *p < 0.05 for indicated comparisons (two-way ANOVA for insulin vs aldehydes). (b) Glucose uptake in L6 cells treated for 30 min with increasing concentrations of 4-HHE. Data are means±SEM, n = 5. *p < 0.05 vs 0 μmol/l 4-HHE + 100 nmol/l insulin (two-way ANOVA). (c) Time course of insulin-stimulated glucose uptake in L6 cells in response to 10 μmol/l 4-HHE. Data are means±SEM, n = 5. *p < 0.05 vs 0 min (one-way ANOVA)