Fig. 2.
Metabolic and inflammatory activation of macrophages in obese adipose tissue is distinct from classical activation by LPS and associates with the presence of type 2 diabetes in obese humans. (a) Enriched or depleted (p < 0.01) KEGG-derived gene sets in ATMs of obese (HFD; n = 4 pools) vs lean (LFD; n = 4 pools) mice. (b, d) Regulation of these enriched or depleted gene sets in LPS-activated (n = 4) vs untreated BMDMs (n = 4) (b) or in CD14+ cells from obese diabetic (n = 6) vs obese non-diabetic individuals (n = 6) (d). Data presented in (b) are derived from GEO accession no. GSE53986 and in (d) from GSE54350. (c) Heat map showing expression of genes involved in inflammation in ATMs from obese vs lean mice presented as log2 ratio (SLR) difference with red representing upregulation and blue representing downregulation in ATMs of HFD- vs LFD-fed mice; *p < 0.05, **p < 0.01 and ***p < 0.001 for obese vs lean mice