Fig. 6.

 Overexpression of IfI202b in C57BL/6J mice increases Zfp423 expression, promotes white adipocyte identity and reduces body temperature. (a) IfI202b was overexpressed in C57BL/6J mice and SCA1-positive adipogenic precursor cells were isolated from WATsc of 8-week-old mice on a standard diet. Cells were grown to confluence, treated with a specific differentiation cocktail up to day 12. Expression levels of indicated genes were analysed by quantitative real-time PCR. (b) Triacylglycerol content of mouse differentiated adipogenic precursor cells (n = 6 per genotype). (c–f) When comparing WATgon from B6-Tg(Ifi202b) mice vs B6-wt control mice (n = 4 mice per genotype), (c) Zfp423 mRNA levels were significantly higher, (d) expression levels of white-selective genes were elevated and (e) mRNA levels of transcription factors and (f) marker genes of thermogenic brown fat determination were reduced. (g) Protein levels of UCP1 in WATgon (20 μg protein) and BAT (2 μg protein) of 8-week-old B6-wt and B6-Tg(Ifi202b) mice. GAPDH was used as a loading control. (h) Ucp1 mRNA expression in BAT of 8-week-old mice. B6-wt, n = 5; B6-Tg(Ifi202b), n = 7. (i) Rectal temperature of 8-week-old mice. B6-wt, n = 8; B6-Tg(Ifi202b), n = 7. White bars, B6-wt; black bars, B6-Tg(Ifi202b). Data are expressed as mean ± SEM *p < 0.05, **p < 0.01 and ***p < 0.001 (Student’s t test). In (c–f), Tle3, p = 0.061; Prdm16, p = 0.106; Ppara, p = 0.116; Ucp1, p = 0.106. ND, not detected