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. 2019 Apr 4;14(4):e0214736. doi: 10.1371/journal.pone.0214736

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© 2019 Tsujioka et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 4 — Sub-cellular localizations of C-terminal actin-binding domains of talin A and talin B. (A) Confocal images of streaming talin B-null cells expressing GFP-I/LWEQ(talB)-PRR-VHP, GFP-I/LWEQ(talB), or GFP-PRR-VHP and talin A-null cells expressing GFP-I/LWEQ(talA)-PRR-VHP. Arrows indicate the direction of migration. GFP-PRR-VHP and GFP-I/LWEQ(talA)-PRR-VHP were also observed in wild-type cells (shown in S2B Fig). (B) Arrested mounds formed by talin B-null cells expressing GFP-I/LWEQ(talB)-PRR-VHP. (C) Immuno-blot analysis using whole cell lysates and an anti-GFP antibody. Marker sizes (kDa) are indicated on the left side of the blot. Scale bars: 10 μm (A) and 200 μm (B).