Table 3.
Cross-reactivity (CR, %) of Various Bile Acids Structurally Related to GCA a
| Analogues | Chemical structure | % CR |
|---|---|---|
| GCA |  |
100 |
| TCA |  |
43.75 |
| GCDCA |  |
1.88 |
| TCDCA |  |
<0.1 |
| GLCA |  |
<0.1 |
| TLCA |  |
<0.1 |
a
The microplate was coated with G1-OVA conjugate at 2 μg/mL. Serial dilutions of GCA or tested compounds in PBS containing 10 % MeOH were mixed with an equal amount of the biotinylated scFv in PBS (1/500, 100 ng/mL). The bound biotinylated scFv was detected with SA-HRP (1/10000, 100 ng/mL). CR is list as a percentage