KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse antibody against HNK1 | DSHB | 1C10; RRID: AB_10570406 |
| Goat anti-Mouse IgG, Secondary antibody, Alexa Fluor 488 | Thermo Fisher | A28175; RRID: AB_2536161 |
| Bacterial and Virus Strains | ||
| Replication-incompetent avian (RIA) retrovirus | Connie Cepko lab, Harvard | https://cepko.hms.harvard.edu |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Alexa Fluor 568 Phalloidin | Thermo Fisher | A12380 |
| DAPI | Thermo Fisher | D1306 |
| Neurobasal media | Thermo Fisher | LS21103049 |
| L-Glutamine | Sigma | G6392 |
| Experimental Models: Cell Lines | ||
| DF1 cells (Chicken embryonic fibroblast cells) | ATCC | CRL-12203 |
| Experimental Models: Organisms/Strains | ||
| Chick. Regular fertilized chicken eggs were obtained from farm and incubated at 37°C to reach the desired Hamburger–Hamilton stage. | AA Lab Eggs, CA, USA | http://www.balutpateros.com |
| Recombinant DNA | ||
| RIA-H2B-GFP-T2A-mCherry | This paper | N/A |
| RIA-membrane-YFP | This paper | N/A |
| RIA-Utrophin-Scarlet | This paper | N/A |
| RIA-PA-Rac-T2A-mCherry | This paper | N/A |
| pTK-NC2-mCherry | Simões-Costa et al., 2012 | https://journals.plos.org/plosgenetics/article?id=10.1371/journal.pgen.1003142 |
| pTK-Sox10E1-EGFP | Betancur et al., 2010 | https://www.pnas.org/content/107/8/3570.long |
| ENV-A | Connie Cepko lab, Harvard | https://www.sciencedirect.com/science/article/pii/S0012160699994320?via%3Dihub |
| VSV-G | Connie Cepko lab, Harvard | https://www.sciencedirect.com/science/article/pii/S0012160699994320?via%3Dihub |