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. Author manuscript; available in PMC: 2019 May 8.
Published in final edited form as: Circulation. 2018 Jan 12;137(19):2052–2067. doi: 10.1161/CIRCULATIONAHA.117.030486

Figure 3. Increased protein acetylation and decreased SIRT3 expression in failing myocardium.

Figure 3.

3A. Increased protein acetylation and decreased SIRT3 expression in heart failure animal model. Whole cell lysates were prepared from heart tissue of sham or TAC or MI mice. Global acetylation level and SIRT3 expression were analyzed by western blotting. GAPDH was used as loading control. 3B. Increased protein acetylation and decreased SIRT3 expression in human samples. Whole cell lysates were prepared from heart tissue of s normal or HF patients. Global acetylation level and SIRTs expression were analyzed by western blotting. GAPDH was used as loading control. 3C. Pathway analysis of top 5 acetylation-related pathway networks in failing myocardium compared to normal. Normal and failing heart tissues were homogenized and the acetylated proteins were enriched by anti-Kac antibody. Statistical analysis of the total spectra counts showed networks with at least 1.5 fold increased in acetylation (p <0.01).