Fig 5. Within the FH models, osteogenic, hypoxia-induced and angiogenic markers were upregulated over time until 48 h of incubation.

Depicted is the relative RNA-expression of the osteogenic markers RUNX2, SPP1, the adipogenic marker PPARG, the hypoxia induced genes LDHA, PGK1, PFKFB3, SLC2A1, and the angiogenic genes VEGFA, and MIF within the in vitro FH models after cultivation in osteogenic differentiation medium for 6, 12, and 48 h under either normoxia (white bars) or hypoxia (black bars). All values are normalized to the “housekeeping gene” B2M and 0 h (Mean ± SEM, n = 3). Statistical analysis was conducted using Mann-Whitney U-test.