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. 2019 Mar 19;8:e43008. doi: 10.7554/eLife.43008

Figure 7. Reb1 slowly exchanges in vivo.

(A) Recovery curves for Reb1 (red), HHT2 (Blue), Sth1 (green), and NHP6A (orange) after photobleaching. Reb1 τ1/2 = 25.8 ± 2.5 s, Sth1 τ1/2 = 7.8 ± 0.7 s, NHP6A τ1/2 = 0.2 ± 0.1 s. (B) Fluorescence images of GFP-labeled Reb1 during a FRAP experiment. The bleached region is indicated with a red circle.

Figure 7.

Figure 7—figure supplement 1. Fluorescence recovery of non-PF proteins in a yeast nucleus.

Figure 7—figure supplement 1.

Fluorescence images of HHT2 (H3), STH1 and NHP6A during a FRAP experiment. Bleached regions are indicated with red circles.