Figure 6. Identification of TCR sequences in library wells and in vivo:

(A) The Vα and Vβ sequences, identified in 5C above from pt 63, were detected, using PCR for the TCR chains, in positive (+) but not negative (−) library wells (selected for IFNγ responses). (DW=control well) There were multiple wells in the CD45RO libraries but a single positive well from the CD45RA+ libraries in which the Vα and Vβ sequences were identified. (B) PBMC isolated in a repeat draw from the same patient were cultured with ZnT8_186–194 peptide. IFNγ⁺CD8⁺ and IFNγ⁻CD8⁺ T cells were identified with a capture assay and sorted. The presence of the TCR Vα and Vβ chains were again identified by PCR (arrow) in the IFNγ+ cells.