Table 1.
Descriptive statistics
| Figure | Sample size | Mean ± SEM | Units |
|---|---|---|---|
| 1I | n = 5 (2 Males, 3 Females) | Level 1: 0.52 ± 0.07 | Proportion of |
| Level 2: 0.48 ± 0.02 | POMC neurons | ||
| Level 3: 0.43 ± 0.02 | |||
| Level 4: 0.45 ± 0.03 | |||
| Level 5: 0.48 ± 0.05 | |||
| 2C | n = 9 | Hypothalamus: 0.51 ± 0.03 | Arbitrary intensity units |
| Restored Group | Striatum: 0.12 ± 0.01 | ||
| 3A | Control: n = 14 | Data order: control, FNΔ2, restored | Grams |
| Males | FNΔ2: n = 8 | Week 3: 9.8 ± 0.6, 11.7 ± 0.7, 10.7 ± 0.4 | |
| Restored: n = 22 | Week 4: 14.4 ± 0.9, 16.4 ± 0.8, 15.4 ± 0.5 | ||
| Week 5: 19.2 ± 0.8, 21.8 ± 1.0, 19.7 ± 0.4 | |||
| Week 6: 21.7 ± 0.6, 25.2 ± 1.3, 21.7 ± 0.3 | |||
| Week 7: 23.2 ± 0.5, 27.6 ± 1.3, 23.1 ± 0.4 | |||
| Week 8: 24.6 ± 0.5, 30.2 ± 1.7, 24.2 ± 0.4 | |||
| Week 9: 25.4 ± 0.5, 32.7 ± 1.7, 24.9 ± 0.4 | |||
| Week 10: 26.9 ± 0.5, 35.1 ± 1.8, 25.7 ± 0.5 | |||
| Week 11: 27.5 ± 0.5, 37.3 ± 1.8, 26.4 ± 0.5 | |||
| Week 12: 28.3 ± 0.5, 39.7 ± 2.0, 27.0 ± 0.4 | |||
| 3B | Control: n = 12 | Data order: control, FNΔ2, restored | Grams |
| Females | FNΔ2: n = 6 | Week 3: 9.8 ± 0.4, 10.9 ± 0.9, 9.6 ± 0.3 | |
| Restored: n = 17 | Week 4: 13.4 ± 0.6, 14.8 ± 1.2, 12.8 ± 0.4 | ||
| Week 5: 16.6 ± 0.5, 19.5 ± 1.2, 15.6 ± 0.3 | |||
| Week 6: 17.5 ± 0.5, 23.0 ± 1.1, 16.6 ± 0.3 | |||
| Week 7: 18.2 ± 0.6, 24.2 ± 1.1, 17.3 ± 0.2 | |||
| Week 8: 19.2 ± 0.7, 26.9 ± 1.3, 17.9 ± 0.3 | |||
| Week 9: 18.6 ± 0.4, 29.0 ± 1.6, 18.5 ± 0.3 | |||
| Week 10: 19.6 ± 0.5, 31.1 ± 1.9, 19.0 ± 0.3 | |||
| Week 11: 20.1 ± 0.5, 34.3 ± 2.2, 19.5 ± 0.3 | |||
| Week 12: 20.4 ± 0.5, 36.1 ± 2.7, 20.0 ± 0.3 | |||
| 3C | Control: n = 8 | 1.55 ± 0.11 | Grams |
| Fat mass, males | FNΔ2: n = 8 | 14.08 ± 1.12 | |
| Restored: n = 10 | 1.52 ± 0.11 | ||
| 3C | Control: n = 8 | 21.36 ± 0.27 | Grams |
| Lean mass, males | FNΔ2: n = 8 | 21.66 ± 0.90 | |
| Restored: n = 10 | 21.20 ± 0.44 | ||
| 3D | Control: n = 7 | 1.46 ± 0.13 | Grams |
| Fat mass, females | FNΔ2: n = 4 | 13.08 ± 1.46 | |
| Restored: n = 10 | 1.60 ± 0.15 | ||
| 3D | Control: n = 7 | 15.67 ± 0.28 | Grams |
| Lean mass, females | FNΔ2: n = 4 | 17.45 ± 1.21 | |
| Restored: n = 10 | 14.88 ± 0.28 | ||
| 3E | Control: n = 9 | 339 ± 11 | Milligrams |
| Gonadal fat, males | FNΔ2: n = 4 | 2111 ± 230 | |
| Restored: n = 10 | 308 ± 18 | ||
| 3E | Control: n = 9 | 189 ± 17 | Milligrams |
| Inguinal fat, males | FNΔ2: n = 4 | 1441 ± 97 | |
| Restored: n = 9 | 206 ± 7 | ||
| 3F | Control: n = 7 | 221 ± 28 | Milligrams |
| Gonadal fat, females | FNΔ2: n = 3 | 2191 ± 243 | |
| Restored: n = 11 | 257 ± 33 | ||
| 3F | Control: n = 7 | 206 ± 14 | Milligrams |
| Inguinal fat, females | FNΔ2: n = 3 | 1532 ± 327 | |
| Restored: n = 10 | 192 ± 15 | ||
| 4F | Three sections/animal | POMC neurons/section | |
| Control, animals | |||
| A568: n = 3 (1 Male, 2 Females) | 82.11 ± 6.67 | ||
| DAB: n = 4 (2 Males, 2 Females) | 122.40 ± 7.04 | ||
| Restored, animals | |||
| A568: n = 3 (2 Males, 1 Female) | 69.22 ± 6.64 | ||
| DAB: n = 3 (2 Males, 1 Female) | 129.30 ± 8.87 | ||
| 4F | Three sections/animal A568: n = 6 (3 Males, 3 Females) DAB: n = 7 (4 Males, 3 Females) A488: n = 5 (2 Males, 3 Females) |
75.67 ± 4.83 125.4 ± 5.45 100.5 ± 4.88 |
POMC neurons/section |
| 5 (data not shown) | Control: n = 6 (4 Males, 2 Females) | 606.30 ± 7.28 | Number of Pomc neurons |
| number of Pomc neurons | Restored: n = 9 (6 Males, 3 Females) | 407.10 ± 14.19 | |
| 5E | Control: n = 6 (4 Males, 2 Females) | Vglut2 +: 55.77 ± 1.86 | % of Pomc neurons |
| Gad67 +: 64.63 ± 0.90 | |||
| Restored: n = 8 (5 Males, 3 Females) | Vglut2 +: 65.80 ± 1.95 | ||
| Gad67 +: 60.59 ± 1.29 | |||
| 5E (data not shown) | Control: n = 6 (4 Males, 2 Females) | –8.87 ± 2.21 | %Vglut2 +, %Gad67 + |
| Difference | Restored: n = 8 (5 Males, 3 Females) | 5.21 ± 2.14 | |
| 5F | Control: n = 6 (4 Males, 2 Females) | Vglut2 +: 338.30 ± 13.65 | Number of Pomc neurons |
| Gad67 +: 391.80 ± 4.21 | |||
| Restored: n = 8 (5 Males, 3 Females) | Vglut2 +: 270.50 ± 12.44 | ||
| Gad67 +: 250.10 ± 12.95 | |||
| 5F (data not shown) | Control: n = 6 (4 Males, 2 Females) | –53.5 ± 12.92 | #Vglut2 +, #Gad67 + |
| Difference | Restored: n = 8 (5 Males, 3 Females) | 20.38 ± 8.87 | |
| 5G | Control: n = 16 (10 Males, 6 Females) | 1.00 ± 0.10 | Relative Pomc expression |
| FNΔ2: n = 4 (1 Male, 3 Females) | 0.03 ± 0.00 | ||
| Restored: n = 15 (7 Males, 8 Females) | 1.07 ± 0.10 | ||
| 6M | n = 6 (3 Males, 3 Females) | Level 1: 16.73 ± 3.10 | % of Pomc neurons |
| Level 2: 25.04 ± 1.41 | |||
| Level 3: 19.90 ± 1.33 | |||
| Level 4: 22.78 ± 2.39 | |||
| Level 5: 15.55 ± 1.04 | |||
| 6N | n = 6 (3 Males, 3 Females) | Pomc-only: 7.05 ± 1.10 | % of Pomc neurons |
| Gad67 +: 34.71 ± 2.42 | |||
| Vglut2 +: 20.62 ± 1.31 | |||
| Vglut2/Gad67 +: 37.61 ± 1.97 | |||
| 6O | n = 6 (3 Males, 3 Females) | Pomc-only: | % of Pomc neurons |
| Level 1: 2.37 ± 0.47 | |||
| Level 2: 1.62 ± 0.37 | |||
| Level 3: 1.28 ± 0.18 | |||
| Level 4: 1.30 ± 0.62 | |||
| Level 5: 0.48 ± 0.35 | |||
| Gad67 +: | |||
| Level 1: 5.44 ± 1.80 | |||
| Level 2: 10.26 ± 0.52 | |||
| Level 3: 8.80 ± 0.71 | |||
| Level 4: 6.47 ± 0.76 | |||
| Level 5: 3.75 ± 0.70 | |||
| Vglut2 +: | |||
| Level 1: 6.22 ± 0.86 | |||
| Level 2: 6.07 ± 1.20 | |||
| Level 3: 3.12 ± 0.31 | |||
| Level 4: 3.90 ± 0.22 | |||
| Level 5: 1.31 ± 0.29 | |||
| Vglut2/Gad67 +: | |||
| Level 1: 2.69 ± 0.54 | |||
| Level 2: 7.09 ± 1.00 | |||
| Level 3: 6.71 ± 0.91 | |||
| Level 4: 11.12 ± 1.58 | |||
| Level 5: 10.02 ± 0.44 | |||
| 6P Linear regression analysis | n = 6 (3 Males, 3 Females) | Pomc-only: y = –6.795x + 40.39 | y = mx + b |
| Gad67 +: y = –1.977x + 25.93 | |||
| Gad67 + (2–5): y = –6.449x + 43.82 | |||
| Vglut2 +: y = –5.784x + 37.35 | |||
| Vglut2/Gad67 +: y = 4.955x + 5.14 | |||