FIGURE 1.

Experimental setup and results. (A) Deamination assay reports AID-catalyzed deaminations on target cassette with multiple trinucleotide motifs NNC embedded in lacZα reporter gene, and examples of mutation patterns where each “T” indicates a C→U mutation. (B) Intrinsic deamination rates for each trinucleotide motif in the (hot hot’)-(hot cold) cassette consisting of a (AAC AGC)₁₅-sss-(AAC GTC)₁₅ sequence. (C) Per-site mutation frequencies observed in experiments. The hot motifs (red) are hotter when amongst hot’ motifs (orange) than amongst cold motifs (blue). (D) A hypothetical path and a set of deamination events along this path, illustrating how the convolution between the scanning dynamics and catalysis of AID generates mutant DNA.