Figure 2.

Mitochondrial NDUFA4L2 was activated by HIF‐1α. Non‐small cell lung cancer (NSCLC) cell lines were cultured in hypoxic conditions. (a) DMOG promoted and 2‐ME inhibited NDUFA4L2 expression. (b,c) Western blotting assays showed that apoptosis of NSCLC cell lines was increased by 2‐ME and inhibited by DMOG. (d–i) The results of quantitative analysis of NDUFA4L2, HIF‐1α, and cleaved caspase‐3 (C‐caspase3) were significant. All data were analyzed as the mean ± standard deviation from at least three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 versus control. DMSO, dimethyl sulfoxide.