Figure 2.
Inhibition of Wnt/β‐catenin pathway impaired ING5 knockdown‐induced invasion of lung cancer cells. (a) Immunofluorescent staining showed that XAV939 significantly decreased the β‐catenin level in both small hairpin (sh)Control and shING5 A549 cells. The mean fluorescence intensity (MFI) values were compared. **P < 0.01, XAV939 group compared to the vehicle control group of shControl A549 cells and shING5 A549 cells, respectively. (
) Vehicle, and (
) XAV939. (b) Western blotting showed that XAV939 significantly decreased the β‐catenin level, with increased p‐β‐catenin. The density of bands was quantified and analyzed. *P < 0.05 and **P < 0.01, XAV939 group compared to the vehicle control group of shControl A549 cells and shING5 A549 cells, respectively. (
) Vehicle, and (
) XAV939. (c) Effects of XAV939 on proliferation of shControl and shING5 A549 cells. **P < 0.001, XAV939 group compared to the vehicle control group of shControl A549 cells. **P < 0.01, XAV939 group compared to the vehicle control group of shING5 A549 cells. (
) shControl+0μMXAV939, (
) shControl+10μMXAV939, (
) shControl+20μMXAV939, (
) shING5+0μMXAV939, (
) shING5+10μMXAV939, and (
) shING5+20μMXAV939 (d) The effects of XAV939 on the colony formation abilities of shControl and shING5 A549 cells. **P < 0.01, XAV939 group compared to the vehicle control group of shControl A549 cells and shING5 A549 cells, respectively. (
) Vehicle, and (
) XAV939. (e) The effects of XAV939 on migration of A549 shControl and shING5 cells by wound‐healing assay. A scratch wound was made on cell surface and cells were photographed at 0, 8, 16, 20, and 24 hours. (f) Effects of XAV939 on Transwell migration of A549 shControl and shING5 cells. The migrated cells were photographed (100 × magnification). *P < 0.05, XAV939 group compared to the vehicle control group of shControl A549 cells and shING5 A549 cells, respectively. (
) Vehicle, and (
) XAV939 (g) The effects of XAV939 on the invasive abilities of A549 shControl and shING5 cells. The invaded cells were photographed (100× magnification). *P < 0.05, XAV939 group compared to the vehicle control group of shControl A549 cells and shING5 A549 cells, respectively. (
) Vehicle, and (
) XAV939. Representative pictures are shown. Data are shown as mean plus standard error of three independent experiments. DAPI, 4′,6‐diamidino‐2‐phenylindole; OD, optical density.