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. 2019 Mar 9;103(8):3521–3535. doi: 10.1007/s00253-019-09618-0

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© The Author(s) 2019

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 1 — Las and rhl QS regulatory genes in P. aeruginosa PAO1 are differentially expressed in a cell density-dependent manner. a Growth of P. aeruginosa in phosphate-limited media (PPGAS). b Transcriptional expression of QS regulatory genes lasR, lasI, rhlR and rhlI. Expression levels were quantified by RT-qPCR, relative mRNA levels for target genes were normalised to the geometric mean of two reference genes (rpoD and proC) and values plotted are the mean calibrator normalised ratios to log phase (6 h). Vertical bars represent S.D. ± (n = 3). Data was analysed using one-way ANOVA followed by Dunnett’s multiple comparison test (**p < 0.01, ***p < 0.001)