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. 2019 Mar 9;103(8):3521–3535. doi: 10.1007/s00253-019-09618-0

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© The Author(s) 2019

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 2 — QS-regulated virulence-associated genes are highly expressed in stationary phase in P. aeruginosa PAO1. Relative transcript levels of virulence-associated rhamnolipid biosynthetic genes arhlA,brhlB, crhlC and exoprotease dlasA and elastase elasB. Relative mRNA levels for target genes were normalised to the geometric mean of two reference genes (rpoD and proC) and values plotted are the mean calibrator normalised ratios to log phase (6 h). Vertical bars represent S.D. ± (n = 3). Data was analysed using one-way ANOVA followed by Dunnett’s multiple comparison test (*p < 0.05, **p < 0.01, ***p < 0.001)