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. 2019 Mar 9;103(8):3521–3535. doi: 10.1007/s00253-019-09618-0

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© The Author(s) 2019

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 3 — Quorum sensing inhibitors (QSIs) significantly reduce expression of las and rhl QS systems in P. aeruginosa. a Molecular structure of the natural QSIs used in this study, salicylic acid (SA), trans-cinnamaldehyde (CA) and positive control furanone C30 (C30F). b Growth of P. aeruginosa with QSIs at sub-MIC concentrations (SA 3.62 mM, CA 2.27 mM and C30F 10 μM). c Relative expression of QS regulatory genes lasR, lasI, rhlR and rhlI with combinations of QSI treatments. Relative mRNA levels for target genes were normalised to the geometric mean of two reference genes (rpoD and proC). Vertical bars represent S.D. ± (n = 3). Data was analysed using two-way ANOVA followed by Bonferroni post-tests (**p < 0.01, ***p < 0.001)