Table 1.
Primers used in the experiments
| Primer name | Primer sequence 5′ to 3′ | Purpose |
|---|---|---|
| cDNA-F/R | F: GCGAGCGAGAGAGATAAGG R: ACTCAAGTAGTAGCCTGAATAC |
cDNA amplification |
| Sub-cloning primers | F: CTggtaccATGGAGCAGAAACTCATCTCTGAAGAGGATATGGAGAGGGGGCCGG R: CTactagtCTACATGGCGTATACTATCTCCCCG |
Sub-cloning in binary vector |
| qRT-PCR-F/R | F: CGACGAGGCTCGCTTCTTTT R: CCCACAGTTGACTTTGGTTGA |
Gene expression level detection in transgenic rice |
| Tubulin | F: TGAGGACTGGTGCTTACCGC R: GCACCATCAAACCTCAGGGA |
Internal control qRT-PCR primers |
| AB-F/R | F: GCGGGGATCTCCGTGTC R: TGCACATACAGATATTCACAGGTT |
Genomic fragment amplification |
| D-F/R | F: GGAGAAGAGGCACCAAGAACAG R: CGCCCTCTCTCCTTATCTCTC |
D promoter region amplification |
| Seq-A-F1 Seq-D-F1 |
F: CGACGCATCTCGCCATC F: GCTGAACTTAAAAGCCCCC |
Sequencing primers for TaSnRK2.9 |
| M13 | F: TGTAAAACGACGGCCAGT R: CAGGAAACAGCTATGACC |
|
| Ta5A1-F/R | F: GCTGAGTGATGTGCCGGTG R: TCGTAGTAATTTTCACTCACCTCCTT |
Chromosomal location check |
| KASP1 | F: GAAGGTGACCAAGTTCATGCTCTTGGCACCAGACCAGAGCCACGGC F: GAAGGTCGGAGTCAACGGATTCTTGGCACCAGACCAGAGCCACGGT R: ACGCATCATCAAACTTGTAAATACC |
KASP assay for SNP at 308 nt (C/T) |
| KASP2 | R: GAAGGTGACCAAGTTCATGCTTGAATGTAGTCCGGAATCGAGTACG R: GAAGGTCGGAGTCAACGGATTTGAATGTAGTCCGGAATCGAGTACT F: CCGAGCTCAACTTTTTCAGAAAA |
KASP assay for SNP at 1700 nt (A/C) |
Small caps italic letters show BamHI (ggtacc) and SpeI (actagt) sites. Underlined letters show 30-bp MYC-tag sequence