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. 2019 Apr 4;9:5632. doi: 10.1038/s41598-019-42001-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cell viability and expression of MHC class I and II, CD80 and CD86 on GL261 cells following cisplatin exposure in vitro. GL261 cells were daily exposed to different doses of cisplatin for 3 days (72 hours). The following day, cells were analysed using flow cytometry for (A) viability using 7AAD staining (percentage of treated viable cells/non-treated viable cells, where the viability of the non-treated cells was regarded as 100%, mean values out of triplicates) or (B). No changes in expression of MHC class I, MHC class II, CD80 and CD86 following cisplatin exposure (1 μM), Histograms from 1 out of 3 experiments are presented.