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. 2019 Apr 4;9:5656. doi: 10.1038/s41598-019-42068-2

Figure 3.

Figure 3

Effect of PX-12 on the enzymatic activity of human Trx1 and PDI. Indicated concentrations of reduced Trx1 or PDI in 50 mM TE buffer and 0.6 mM NADPH was incubated with 20 µM PX-12 for 30 min at 37°C. Then insulin and TrxR were added to a final concentration of 300 µM and 50 nM, respectively, and incubated at 37°C for 20 min. Reaction was stopped by addition of 6 M guanidine-HCl in TE buffer. The absorbance at 412 nm was read against a blank without Trx or PDI. Representative experiment out of several is shown.