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. 2019 Apr 4;9:5656. doi: 10.1038/s41598-019-42068-2

Figure 6.

Figure 6

PX-12, but not TE-2, interferes with tubulin acetylation. 80% confluent TZM-bl cells were exposed to 25 µM colchicine (de-acetylating control), 3 µM paclitaxel (acetylating control), medium only (normal control) or 100 µM, 10 µM or 1 µM PX-12 for 30 min before 1 µg/ml gp120 was added to indicated samples and the cells were then further incubated to a total of 18 h. Western blot was performed on the samples with an antibody against acetylated tubulin and an antibody against actin (loading control). The membrane was developed using the SYNGENE system. The experiment was repeated four times and the results shown are representative of the experiments.