FIGURE 3.

Presence of lactonase reduces biofilm formation of the microbial community in the bioreactors. (A) Bioreactor parameters over the time course of the experiment (21 days). Measurements (duplicates) were performed on the three distinct bioreactors equipped with different filtration cartridges: the 2× lactonase cartridge, containing only lactonase beads (blue line), the control cartridge containing only control beads (dark line), and the 1× lactonase cartridge containing a 1:1 ratio of lactonase beads and control beads (green line). Biofilm quantification in submerged wells as quantified by crystal violet binding measured at 550 nm. (B) Glass slips submerged in the bioreactors were stained using SYBR gold DNA stain and visualized using a 60× magnification.