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. 2019 Mar 29;10:606. doi: 10.3389/fimmu.2019.00606

Figure 6.

Figure 6

T-bet regulates TFH cell proliferation and apoptosis. WT or Tbx21−/− SMARTA cells (CD45.1+) were transferred into naïve WT mice (CD45.2+), which would be infected with LCMV. Splenocytes were isolated and analyzed for TFH response at day 2, 5, and 8 post infection. (A) Flow cytometry of BrdU incorporation in TFH cells (left), and summary of BrdU-incorporated cells percentage (right). Numbers adjacent to outlined areas indicate percent of each cell subset in parent subset. (B) Flow cytometry of Annexin-V and 7-AAD staining in TFH cells (left), and summary of Annexin-V+7-AAD− percentage (right). Numbers in quadrants indicate percent cells in each. (C) Flow cytometry of BCL-2 expression in TFH cells (left), and the summary of Bcl-2 expression (showed as MFI) of TFH cells. ns, not significant; **P < 0.01, ***P < 0.001, ****P < 0.0001 (unpaired two-tailed t-test). Data are representative of two independent experiments with 3–5 mice per group (error bars, SEM).