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. 2019 Mar 29;10:606. doi: 10.3389/fimmu.2019.00606

Figure 7.

Figure 7

T-bet dependency of the TFH and TH1 cell transcriptomes. (A) Clustering of genes based on their expression in WT TH1, WT TFH, Tbx21−/− TH1, and Tbx21−/− TFH cells. (B) Principal component analysis (PCA) of genes in the five cell populations listed in (A). (C) Venn diagram comparing the differentially expressed genes in TH1 and TFH cells. The red numbers represent upregulated genes, and the green numbers represent downregulated genes. (D) Heat map of genes related to cell proliferation in WT TH1, Tbx21−/− TH1, WT TFH, and Tbx21−/− TFH cells. (E) GSEA of WT and Tbx21−/− TH1 genes showing gene sets upregulated in TH1 cells relative to TFH cells (left) and gene sets upregulated in TFH cells relative to TH1 cells (right). (F) GSEA of WT and Tbx21−/− TFH genes showing gene sets upregulated in TH1 cells relative to TFH cells (left) and gene sets upregulated in TFH cells relative to TH1 cells (right). The gene sets used in GSEA include published data (GEO accession code GSE21379) and our microarray data (GEO accession code GSE122931). (G) Heat map of genes related to T cell differentiation and function in WT TH1, Tbx21−/− TH1, WT TFH, and Tbx21−/− TFH cells.