Table 2.

Primers used for real-time PCR quantification.

Target bacteria	Primer	Tm size	Product	References
		(°C)	(bp)
General Bacteria 16Sr DNA	F: 5′- CGGCAACGAGCGCAACCC-3′	58	130	Egan, 2005
	R: 5′-CCATTGTAGCACGTGTGTAGCC-3′
Ruminococcus albus	F: 5′-CCCTAAAAGCAGTCTTAGTTCG-3′	54	176	Khafipour et al., 2009
	R: 5′-CCTCCTTGCGGTTAGAACA-3′
Ruminococcus flavefaciens	F: 5′-CGAACGGAGATAATTTGAGTTTACTTAGG-3′	58	132	Khafipour et al., 2009
	R: 5′-CGGTCTCTGTATGTTATGAGGTATTACC-3′
Fibrobacter succinogenes	F: 5′-GGAGCGTAGGCGGAGATTCA-3′	59	97	Khafipour et al., 2009
	R: 5′-GCCTGCCCCTGAACTATCCA-3′
Butyrivibrio fibrisolvens	F: 5′-ACCGCATAAGCGCACGGA-3′	59	124	Khafipour et al., 2009
	R: 5′-CGGGTCCATCTTGTACCGATAAAT-3′