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. 2019 Mar 29;10:388. doi: 10.3389/fpls.2019.00388

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Copyright © 2019 Timerbaev, Mitiouchkina, Pushin and Dolgov.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Schematic representation of the pMF-E8 vector T-DNA region with primer set positions. RS, recombination site; RB and LB, right and left border of T-DNA, respectively; 35S, CaMV35S promoter; CodA-nptII, translational fusion gene for positive (nptII) and negative (codA) selection; nos, nopaline synthase terminator; Rec-LBD, translational fusion of Recombinase R-LBD; E8 prom, E8 promoter; Thau, thaumatin II; rbcS3A, rbcS3A terminator. For interpretation of primer set numbers, see Table 1. HindIII, position of the restriction site for which the DNA was digested for the Southern blot assay.