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Semiquantitative RT-PCR analysis of thaumatin II gene expression in marker-free apple plants. In the upper panel the thaumatin gene is shown, in the lower panel the housekeeping actin gene. M, molecular weight marker; H2O, water; p, plasmid pMF-E8 (used for plant transformation); NT, non-transgenic control; L1, L2, and L6, parent transgenic lines; other numbers, marker-free sublines (except 1-1).
