Table 1.

Primer sequences and amplicon sizes.

Acronym	Gene or combination	Forward/reverse primer sequences (5′–3′)	Amplicon size (bp)	Method
E8-prom	E8 (promoter)	CTATCCCGGGAGGCGCGCCAGAAGGAATTTCACGAAATC/TCAGGATCCCTTCTTTTGCACTGTGAATG	1146	PCR (cloning)
3A-ter	Ribulose-1,5-bisphosphate carboxylase/oxygenase (rbcS3A) terminator	ACTGACCGGTTCTAGAAAAACTAATTGCC/ACTCCTGCAGGCGAGGGAGTAGTAGAGATAAG	423	—»—
Thau-CDS	Thaumatin II (CDS)	TAGGATCCATGGCCGCCACCAC/ACTACCGGTTTACTCGTCTTCAAGTTCAAG	725	PCR (cloning), Southern blotting
(1) RS site	RS site-35S promoter	CGATTTGATGAAAGAATGAATTAATG/GTGTGTCGTGCTCCACCATG	526	PCR
(2) CodA	CaMV35S promoter- Cytosine deaminase	CCAACCACGTCTTCAAAGCA/AATGCCTTCAAACAGCGTGC	589	—»—
(3) NptII	Neomycin phosphotransferase II	TCTGATGCCGCCGTGTTCC/ATGCGCGCCTTGAGCCTG	440	PCR, RT-PCR, Southern blotting
(4) Nos ter	Nopaline synthase terminator	CCGATCGTTCAAACAT/GTAACATAGATGACACCGCG	249	PCR
(5) 35S prom	CaMV35S promoter	AGCACGACACACTTGTCTACTC/CTCTCCAAATGAAAT	406	PCR
(6) RecR	Recombinase R	ATGCGCAAGGAGGCAGGTCG/GCCACACGGGAGACGCCTTC	637	PCR, RT-PCR, Southern blotting
(7) Thau	Thaumatin II	GCGCTGCCACCTTCGAGATCG/GCAGGTGACGGTGGTTGGCT	584	PCR, RT-PCR
(8) E8-Thau	E8 promoter-thaumatin II	CTTAATCAGACGTATTGGGTTTC/AGCCTTTGATGTTGGAGATGTC	624	PCR
(9) Thau-ter	Thaumatin II-rbcS3A terminator	GCTCAACCAGTACGGCAAGG/CAAGGGAAAACCCAAAGGAG	445	—»—
Actin	Actin 11	TCATCATACTCGGCCTTCGC/CCATCCATGATTGGAATGGAAGC	302	RT-PCR
THFS	Formate-tetrahydrofolate ligase	AGCAGCGTTGAATACTCAGAG/ATACTGGGTTTTCGCCATGC	99	Real-time PCR
TMp1	Type 1 membrane protein-like	AGACCGACTCAATGTTGCTCTC/GTGGAAGGTGGTGCAAATCC	73	—»—
ACT11	Actin 11	GCTGTTCTTTCCCTCTACGC/GCATGGGGAAGAGCATATCC	110	—»—
Th84	Thaumatin II	AGAGTCCTGGACCATCAAC/CCGCTGTCGTCGAAATAG	84	—»—
Th113	—»—	GCACCGTGTTCCAGACGAG/GTCCAGGACATAACTGAACGC	113	—»—
Th95	—»—	AGCTCAACTCGGGAGAGTC/GCTGTCGTCGAAATAGCAGTC	95	—»—