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Marker-free apple obtaining experimental options.
| Code | Name | Induction, 10 μM Dex | Selection, 250 mg L-1 5-FC (immediate) | Selection, 250 mg L-1 5-FC (delayed for 7 days) | Intent |
|---|---|---|---|---|---|
| 0-0 | Without selection | - | - | - | Background regeneration |
| 0-2 | Delayed selection | + | - | + | DNA excision, selection begins after 7 days |
| 250-0 | Without induction | - | + | + | Background DNA excision |
| 250-2 | Immediate selection | + | + | + | DNA excision; impact of 5-FC and Dex on regeneration |
