Table 4.
Selection of putative marker-free regenerants.
| Event | Variant | Line | Number of explants | Number of regenerants | Regeneration efficiency, % | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Initially1 | Before regeneration2 | Cut3 | Rooted4 | Primary5 | Final6 | ||||||||
| I | 0-2 | 1 | 127 | 101 | 8 | 1 | 7.9 | 1 | |||||
| 6 | 226 | 198 | 35 | 11 | 17.7 | 5.6 | |||||||
| 250-0 | 1 | 159 | 159 | 2 | 0 | 1.3 | 0 | ||||||
| 6 | 161 | 161 | 32 | 2 | 19.9 | 1.2 | |||||||
| 250-2 | 1 | 155 | 152 | 27 | 4 | 17.8 | 2.6 | ||||||
| 6 | 180 | 162 | 48 | 13 | 29.6 | 8 | |||||||
| 0-0 | NT | 159 | 104 | 78 | – | 75 | – | ||||||
| 250-2 | 142 | 119 | 66 | – | 55.5 | – | |||||||
| II | 250-0 | 6 | 253 | 53 | 12 | 2 | 22.6 | 3.8 | |||||
| 250-2 | 98 | 74 | 17 | 12 | 23.0 | 16.2 | |||||||
| 0-0 | NT | 66 | 31 | 14 | – | 45.2 | – | ||||||
| 250-2 | 75 | 37 | 10 | – | 27.0 | – | |||||||
| Total number of obtained regenerants | 45 | ||||||||||||
1total number of cut explants; 2remaining number of explants after two passages; 3number of shoots separated from the explant for rooting; 4number of rooted shoots; 5ratio of cut shoots to the total number of explants before regeneration; 6ratio of rooted shoots to the total number of explants before regeneration.