Table 1.
Final ranking of candidate reference genes from dsRNA-treated beetles according to values given by GeNorm, NormFinder, BestKeeper, and delta Ct and a comprehensive ranking by RefFinder.
| Gene | GeNorm | NormFinder | BestKeeper | delta-CT | Comprehensive | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| M | R | SV | R | SD | R | SD | R | GM | R | |
| rps18 | 0.028 | 1 | 0.81 | 7 | 0.65 | 1 | 0.94 | 5 | 2.43 | 1 |
| ef1a | 0.028 | 1 | 0.773 | 6 | 0.67 | 2 | 0.92 | 4 | 2.63 | 2 |
| rpl13 | 0.617 | 6 | 0.336 | 3 | 1.44 | 7 | 1.08 | 7 | 5.66 | 7 |
| ak | 0.501 | 5 | 0.165 | 1 | 1.41 | 6 | 0.97 | 6 | 3.83 | 6 |
| sdf | 0.1 | 2 | 0.666 | 5 | 0.71 | 3 | 0.87 | 3 | 3.41 | 5 |
| rpl32 | 0.326 | 4 | 0.206 | 2 | 1.11 | 5 | 0.84 | 1 | 2.66 | 3 |
| tub | 0.177 | 3 | 0.546 | 4 | 0.79 | 4 | 0.86 | 2 | 3.36 | 4 |
| ubiq | 1.16 | 7 | 2.777 | 8 | 3.1 | 8 | 2.79 | 8 | 8 | 8 |
M: gene expression stability; R: ranking; SV stability value; SD: standard deviation; GM: Geomean value.