. 2019 Apr 4;9:5640. doi: 10.1038/s41598-019-42072-6

Table 2.

Candidate reference genes and corresponding primer sequences.

Gene Name	Sequence 5′3′	R²	Eff%
rps18 – Ribosomal Protein S18	GCCCTCTTGTTCAAATCCAC	0.99	98.6
rps18 – Ribosomal Protein S18	CTTAACGGCCATCAAAGGAG	0.99	98.6
ef1a – Elongation Factor – 1 alpha	TCCAAGAGGTGGGAATTCAG	0.99	100
ef1a – Elongation Factor – 1 alpha	GATCGTCGTTCAGGAAAAGC	0.99	100
rpl13 – Ribosomal Protein L13	AACCCCAAGAGGAAAGGATG	0.99	96.2
rpl13 – Ribosomal Protein L13	CCAGGCGCTTTTTAGAACTG	0.99	96.2
ak – Arginine Kinase	GATGGGGTCGAACAAATCAG	0.99	98.3
ak – Arginine Kinase	AGAAAACGTCCTTCGGTTCC	0.99	98.3
sdf – Succinate dehydrogenase flavoprotein subunit A	AGTTGGCAGAGACCCATTTG	0.99	100
sdf – Succinate dehydrogenase flavoprotein subunit A	CGCGTGTCGAGATGTTAAAGC	0.99	100
rpl32 – Ribosomal Protein L32	ATTGTGGACCAGCACTTTCC	0.99	99.3
rpl32 – Ribosomal Protein L32	TATCGACAACAGGGTGAGGAG	0.99	99.3
tub - Tubulin	TCCTGATCCTGTCCAAAACC	0.99	99.3
tub - Tubulin	TGATCACCGGAAAGGAAGAC	0.99	99.3
ubiq -Ubiquitin	ATTGGAAGATGGACGCACTC	0.99	95.7
ubiq -Ubiquitin	TGCCAGTCAAACTCTTCACG	0.99	95.7

R²: Correlation Coefficients; Eff: Amplification efficiency.