Table 3.
Primer sequences and amplicon size for qPCR and dsRNA synthesis of the target genes hsp, shi, and iap.
| Gene | Primer | Primer Sequence (5′-3′) | Amplicon (bp) |
|---|---|---|---|
| hsp – heat shock protein | F-qRNA-HSP | ACACGCACACTCGTTCTCAC | 139 |
| R-qRNA-HSP | TACGCGTACTCGCTGAAGAA | ||
| F-dsRNA-HSP |
TAATACGACTCACTATAGGGGTGCAG CAACTGGTCAAAGA |
351 | |
| R-dsRNA-HSP |
TAATACGACTCACTATAGGGGTCTTT GGTCATGGGACGTT |
||
| shi – shibire | F-qRNA-SHI | AGTTCGCCGTTGATGAAATC | 86 |
| R-qRNA-SHI | TCGAGCAGGGCTTTATGTCT | ||
| F-dsRNA-SHI |
TAATACGACTCACTATAGGGTAGATC GGTGTCAGTTCCCC |
379 | |
| R-dsRNA-SHI |
TAATACGACTCACTATAGGGGCGAGC GCGTTTTCTATTAC |
||
| iap – inhibitor of apoptosis | F-qRNA-IAP | TTTCGTTTGATGCTCGACTG | 109 |
| R-qRNA-IAP | TCTTCGCCTGTCCTGTCTTT | ||
| F-dsRNA-IAP |
TAATACGACTCACTATAGGGGTCCCGC TCATCCAGATAAA |
370 | |
| R-dsRNA-IAP |
TAATACGACTCACTATAGGGTTTTGCCT CTTTCGCACTTT |
Bold letters denote promoter sequences of T7 RNA polymerase.