REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
AlexaFluor® 488 Phalloidin	Invitrogen	A12379; RRID:AB_2315147
AlexaFluor® 647 anti-CD107a clone H4A3	Biolegend	328612; RRID:AB_1227506
AlexaFluor® 647 anti-perforin clone dG9	Biolegend	308109; RRID:AB_493255
PE/CY7 anti-CD107a antibody clone H4A3	Biolegend	328617; RRID:AB_11147761
PE anti-CD3 clone HIT3a	Biolegend	300307; RRID:AB_314043
V500 anti-CD8 clone RPA-T8	BD Horizon	560775; RRID:AB_1937333
BV605 anti-CD4 clone OKT4	Biolegend	317437; RRID:AB_11204077
Pacific blue anti-Perforin clone B-D48	Biolegend	353305; RRID:AB_11124346
APC anti-IFN-γ clone B27	Biolegend	506510; RRID:AB_315443
FITC anti-TNF-α clone MAb11	BD PharMingen	554512; RRID:AB_395443
PerCP-Cy5.5 anti-MIP1-β clone D21-13551 RUO	BD PharMingen	560688; RRID:AB_1727567
AlexaFluor®700 anti-GranzymeB clone GB11	BD PharMingen	561016; RRID:AB_2033973
Purified anti-human CD11a clone 38	Genetex	GTX26132; RRID:AB_380799
Anti-human CD18 clone CBR LFA-1/2	Biolegend	366302; RRID:AB_2565276
Anti-human ICAM-2 clone CBRIC2/2	Genetex	GTX42521; RRID:AB_11169501
Anti-human LFA3 clone TS2/9	Biolegend	330912; RRID:AB_2075983
Ultra-LEAF™ purified anti- HLA-A, B, C	Biolegend	311428; RRID:AB_2561492
Chemicals, Peptides, and Recombinant Proteins
Probenecid	Sigma-Aldrich	P8761
CellTracker™ Orange CMTMR dye	ThermoFisher	C2927
ProLong™ Gold Antifade mountant	ThermoFisher	P10144
GolgiStop™	BD Biosciences	554724
GolgiPlug™	BD Biosciences	555029
Fixable blue viability dye	Life Technologies	L-23105
BD Cytofix/Cytoperm™	BD Biosciences	554722
Efavirenz	Sigma	SML0536
Epoxomicin	Enzo Life Science	BML-PI127-0100
Bestatin	Sigma Aldrich	B8385
E64	Enzo Life Science	ALX-260-007
BsaJI	NEB	R0536L
Critical Commercial Assays
CCF2-AM loading kit	Invitrogen	K1025
Dynabeads Human T-Activator CD3/CD28	ThermoFisher	111.31D
EasySep Human CD4+ T cell enrichment	Stemcell	19052
Alliance HIV-1 P24 ANTIGEN ELISA Kit	Perkin Elmer	NEK050001KT
Gentra Puregene cell kit	QIAGEN	158745
Experimental Models: Cell Lines
HeLa cells	ATCC	CCL-2
HEK293T cells	ATCC	CRL-3216
Recombinant DNA
Plasmid: NL4.3-GFP-X4	T.Murooka T. Mempel	N/A
Plasmid: NL4.3-GFP-R5	T.Murooka T. Mempel	N/A
Plasmid: pMM310	NIH	11444
Software and Algorithms
IMARIS	Bitplane	http://www.bitplane.com/imaris
GraphPad Prism	GraphPad Software Inc.	https://www.graphpad.com/scientific-software/prism/
FlowJo	FlowJo LLC	https://www.flowjo.com/
Quantalife ddPCR software	QuantaLife, Inc.	http://www.bio-rad.com/