Figure 2. Stability of AT in the absence and presence of small molecules.

Relative ellipticity change at 222 nm is plotted against temperature over the range of 35–80°C of 3 µM of AT in the absence and presence of 1 M trehalose, 1.5 M sorbitol and 1.5 M mannose as described in methods. Relative ellipticities were calculated by using the relation [θ_obs− θ_min]/[θ_{max −}θ_min] [38] where θ_min and θ_max were fitted values of ellipticity at the lowest and highest temperatures used in the study, respectively, and θ_obs is the observed ellipticity at temperature T. Each curve is an average of three experiments.