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. 2019 Apr 5;39(4):BSR20182259. doi: 10.1042/BSR20182259

Figure 4. Effect of trehalose on native state of AT.

Figure 4

(A) Bis-ANS fluorescence, (B) Far-UV CD and (C) Near-UV CD spectra of native AT in the absence and presence of 1 M trehalose. Experimental details are mentioned in Materials and methods section. All measurements were carried out at 25°C in 20 mM phosphate buffer containing 100 mM NaCl and 0.1% EDTA (PNE buffer). The concentration of protein was 1–20 µM and that of trehalose was 1 M. All the data were corrected for buffer effect. Each curve represents an average of three (for Bis-ANS and Far-UV CD) and two (for near-UV CD) independent experiments.