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. 2019 Apr 5;39(4):BSR20182259. doi: 10.1042/BSR20182259

Figure 6. Effect of trehalose on intermediate state of AT.

Figure 6

(A) Far-UV CD spectra and (B) Bis-ANS fluorescence of AT in the absence and presence of 1 M trehalose was done to observe conformation of intermediate state. AT was incubated with 2 M GdnHCl for 2 h in the absence and presence of 1 M trehalose prior to CD and Fluorescence analysis. The concentration of AT was 1 µM and the molar ratio of AT to bis-ANS was 1:10. Data were corrected by subtracting the buffer contribution. All data points in each plot were obtained from the average value of atleast three independent experiments.