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. 2019 Apr 5;39(4):BSR20181038. doi: 10.1042/BSR20181038

Figure 2. Knockdown of lncRNA HOTAIR increased cellular sensitivity to IR.

Figure 2

A siRNA was used to knock down HOTAIR in MCF7 cells (A). Cell proliferation was measured by using a CCK8 assay in wild-type and HOTAIR knockdown cells (B). We measured cell apoptosis in MCF7 cells and HOTAIR knockdown cells using an Annexin V and PI double staining kit (C and D). (E) Cell survival data in irradiated normal control cells and HOTAIR knockdown cells. (F) Representative images of Western blot analysis of bax and caspase 3 with/without radiation treatment in normal and HOTAIR knockdown cells. *P<0.05, **P<0.01 vs the control groups.