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. 2019 Apr 5;39(4):BSR20190072. doi: 10.1042/BSR20190072

Figure 5. Fatty acid distribution in major lipid classes reveals striking differences between oleate versus palmitate and LPS treatments.

Figure 5

BV2 cells were treated for 24 h with 0.25% (37.8 µM) BSA, 200 µM oleate, 200 µM palmitate or 1 µg/ml LPS followed by lipidomic analysis. PUFA incorporation into (A) TAG, (B) PE, (C) pPE, and (D) PC. (E) Ratio of long chain and very long chain fatty acid-enriched ceramides. (F) GSH/GSSG ratio. Values represent averages ± SEM from two (F) or six (A–E) independent experiments and were compared using one-way ANOVA followed by Tukey’s post test. * P<0.05 vs BSA. #P<0.05 vs LPS. $P<0.05 vs OA.

Abbreviations: ARA, arachidonic acid; DHA, docosahexaenoic acid; DPA, docosapentaenoic acid; OA, oleate; PA, palmitate; PC, phosphatidylcholine; PE, phosphatidylethanolamine; pPE, plasmalogens of phosphatidylethanolamine.