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. 2019 Mar 11;13(2):95–104. doi: 10.4162/nrp.2019.13.2.95

Fig. 3. Effect of LF on expression of IL8 and XBP1s by ER stress in polarized human intestinal epithelial cell.

Fig. 3

Polarized Caco-2 cells was pretreated with indicated concentrations of LF for 24 h, followed by 3 µM TG stimulation apically for additional 4 h (A and B) or 1 h (C and D). Total RNA was extracted and cDNA prepared for real-time qPCR. Human IL8 (A, B) and XBP1s (C, D) were normalized by human GAPDH. The fold change of IL8 or XBP1s expression induced by TG was calculated at each LF concentration (B, D). The experiments were performed individually at least three times. Data are expressed as mean ± SD. The significant differences between the LF concentrations were analyzed by One-way ANOVA. The TG effect at each concentration was analyzed by student's t-test. *P < 0.05. IL8, interleukin 8; XBP1s, X-box binding protein 1 spliced form; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; TG, triglyceride; LF, L. chinense fruit.