Fig. 4. Effect of LF on proinflammatory response in MEF cells.

Wt MEF (A and B), XBP1 KO MEF (C and D) and IRE1α KO MEF (E and F) cell lines were pretreated with LF for 24 h, followed by 3 µM TG for additional 4 h. Total RNA was extracted and cDNA prepared for real-time qPCR. mRNA expression of mouse IL6 (A, C and E) and the fold change of IL6 expression induced by TG (B, D and F) were normalized by mouse β-actin. The experiments were performed individually at least three times. Data are expressed as mean ± SD. Significant differences between the different concentrations were analyzed by One-way ANOVA. The TG effect at each concentration was analyzed by student's t-test. ^*P < 0.05. IL6, interleukin 6; LF, L. chinense fruit; TG, triglyceride; XBP1s, X-box binding protein 1 spliced form.