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. 2018 Oct 25;133(3):224–236. doi: 10.1182/blood-2018-08-867648

Figure 1.

Figure 1.

Cholinergic neural signals regulate circadian traffic of HSCs and leukocytes in mice. (A) AChE activity in urine samples from Gfra2−/− and WT mice collected in the nocturnal (black) and diurnal (yellow) periods. HSPCs measured as CFU-Cs (B) and WBCs (C) in peripheral blood of Gfra2−/− mice and control Gfra2+/− mice harvested at the specified ZT (hours after light onset). ZT1 has been duplicated to facilitate viewing. (D) HSCs, measured by long-term competitive repopulation assay, in peripheral blood harvested at ZT13 from Gfra2−/− mice and control Gfra2+/− mice. The log fraction of mice that failed reconstitution is plotted against the transplanted blood volume using ELDA software.59 Likelihood ratio test of single-hit model, P = .006, χ2 test. Blood HSC concentrations are indicated (n = 5). Data are mean ± standard error of the mean; n (inside bars/symbols) and P values (multivariate analysis for >2 groups) are indicated. *P < .05, ***P < .001, 1-way ANOVA and Bonferroni comparisons (A), multiple 2-tailed test (B-C), χ2 test (D).